prl r  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: Elevated high-mannose N-glycans hamper endometrial decidualization

doi: 10.1016/j.isci.2023.108170

Figure Lengend Snippet:

Article Snippet: 5′-CGATTCGGCACTTCAGGAGCTT-3′ , Sangon Biotech , PRL-R.

Techniques: Microarray, Bicinchoninic Acid Protein Assay, Recombinant, Plasmid Preparation, Software

Journal: PLoS ONE

Article Title: Identification of an Internal RNA Element Essential for Replication and Translational Enhancement of Tobacco Necrosis Virus A C

doi: 10.1371/journal.pone.0057938

Figure Lengend Snippet: (A) Schematic illustrations of firefly luciferase (F-Luc) expression vectors for transcription of chimaeric RNAs and protoplast transfections are shown above. The full-length F-Luc gene used for expression is represented by white boxes interrupted by two lines to reduce the length of the illustrations. The gray boxes represent TNV-A C fragments of different lengths that are indicated by their nucleotide positions relative to TNV RNA. The pentagons represent the T7 promoters and the solid lines indicate poly (A) tails of the mRNA transcripts. The designations upstream of the Luc sequences are: TEV Leader = TEV leader sequences involved in translational enhancement, RN = 81 nonviral nucleotides derived from the plasmid pGL3-Basic, 5′ UTR = TNV-A C 5′ UTR sequence, 3′ UTR = TNV-A C 3′ UTR sequence. Roman numerals I to IV represent the construct categories for the uncapped mRNA transcripts used for F-Luc expression. (B) Relative F-Luc expression in BY-2 protoplasts was assayed as described in the text, and the results represent an average of three independent experiments, each of which was carried out in triplicate. Expression of the Renilla luciferase (R-Luc) from the plasmid pRL-TK served as an internal control throughout the experiments. (C) DNA plasmids for transient expression of firefly luciferase (F-Luc) are illustrated, and the symbols have the same meanings as those in (A), except that the pentagon represents the 35S promoter, and the Cauliflower mosaic virus terminator sequences are indicated. (D) Relative F-Luc expression detected as in (B).

Article Snippet: All of the derivatives were verified by cDNA sequencing, and a Renilla luciferase (R-LUC) gene from pRL-TK (Promega) driven by T7 or 35S promoter was used as a control throughout the experiments.

Techniques: Luciferase, Expressing, Transfection, Derivative Assay, Plasmid Preparation, Sequencing, Construct

Journal: PLoS ONE

Article Title: MICA-G129R: A bifunctional fusion protein increases PRLR-positive breast cancer cell death in co-culture with natural killer cells

doi: 10.1371/journal.pone.0252662

Figure Lengend Snippet: The cytotoxicity of NK-92 cells on T-47D cells was measured at different conditions. A. T-47D cells were co-cultured with NK-92 cells at different effector/target ratios 5:1, 2:1, 1:1, and 1:2 for 24 hours with control or MICA-G129R conditioned media. B. The co-culture of T-47D cells and NK-92 cells at the ratio of 1:1 was treated with the control, MICA-G129R, MICA or G129R conditioned media for 24 hours. C. The NK-92 cells were co-cultured with T-47D cells, HeLa cells (PRLR-negative), 293 (PRLR-negative) or PRLR ectopic expressed 293 cells at the ratio of 1:1 with control or MICA-G129R conditioned media for 24 hours. Data are presented as mean ± SD (n = 3). * indicates P<0.05; ** indicates P<0.01.

Article Snippet: The following antibodies were used in Western blot: monoclonal mouse anti-human MICA (sc-137242), PRL (sc-46698), PRLR (sc-377098) antibodies, mouse IgG light chain binding protein conjugated to HRP (mIgG BP-HRP) (sc-516102) as secondary antibody (purchased from Santa Cruz Biotechnology, CA, USA) and monoclonal mouse anti-V5 antibody (#80076) (purchased from Cell Signaling Technology, MA, USA).

Techniques: Cell Culture, Control, Co-Culture Assay